Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (135,145)

Singleplex and Multiplex Bead Based Immunoassays (6,198)

ELISA Substrates and Reagents (2,230)

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211 – 225 of 141,015 results

211

Invitrogen™ Mouse IgM Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgM
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16019
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin M

212

Invitrogen™ Mouse IgA Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgA
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	238447
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin A

213

Invitrogen™ Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Description	Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA Kit
Interassay CV	8.4%
Intraassay CV	7
Total Assay Time	2 hrs 30 mins
Sample Volume	Fecal Extract 50 μL, Plasma 1 μL, Saliva 25 μL, Serum 1 μL, Supernatant 50 μL, Urine 25 μL
Assay Sensitivity	90.9 pg/mL
Storage Requirements	-20°C
Research Discipline	Reproductive Biology
Assay Range	96 to 60,000 pg/mL
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hrs 20 mins
Detection Method	Absorbance at 450 nm
Regulatory Status	RUO
Target	DHEA-S
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye	HRP

214

Invitrogen™ pNPP Solution

Suitable for use in immunoassays

Pricing & Availability

215

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

Pricing & Availability

216

Invitrogen™ Human HE4/WFDC2 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	WFDC2
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.25 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.25 to 25 ng/mL
Test Time	1 hrs 20 mins
Accession Number	1600023A02Rik; dJ461P17.6; EDDM4; epididymal protein 4; epididymal secretory protein 4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; He4; major epididymis-specific protein E4; MGC57529; Putative protease inhibitor WAP5; RE4; WAP domain containing protein HE4-V4; WAP domain-containing protein HE4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WFDC2
Regulatory Status	RUO
Target	WFDC2
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	10406
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

217

Invitrogen™ Multispecies ERK1/ERK2 (Total/Phospho) InstantOne™ ELISA Kit

ELISA

Pricing & Availability

218

Promotions Available

R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

Human IL-1 beta/IL-1F2 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human IL-1 beta
Target Species	Human
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3553
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Sample Volume	Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL)
Synonym	catabolin, IL-1, IL1 beta, IL-1 beta, IL-1 b, IL1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Assay Sensitivity	1pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	3.90 - 250pg/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

219

Invitrogen™ Human ANGPTL3 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	ANGPTL3
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	8.2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	8.2 to 2,000 pg/mL
Test Time	1 hrs 20 mins
Accession Number	ANG-5; angiopoietin 5; angiopoietin like 3; Angiopoietin-5; angiopoietin-like 3; angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGPT5; Angptl3; ANGPTL3(17-221); ANGPTL3(17-224); ANL3; FHBL2; hypl; hypolipidemia; UNQ153/PRO179
Regulatory Status	RUO
Target	ANGPTL3
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	27329
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

220

Invitrogen™ Mouse IL-17A (homodimer) Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	AV080368,Mcp-1
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	Q62386
Regulatory Status	RUO
Target	IL-17 A
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This Uncoated ELISA Kitmay contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16171
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-17 A, IL-17 AA

221

Promotions Available

R&D Systems™ Human IFN-gamma Quantikine ELISA Kit

solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma.

Pricing & Availability
Specifications

Target	IFN-gamma
Specificity	Natural and Recombinant Human IFN-gamma
Target Species	Human
Gene ID (Entrez)	3458
Sample Type	Cell Culture Supernatant,Plasma (EDTA),Heparin Plasma,Serum
Synonym	IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma
Assay Sensitivity	5.69 pg/mL
Storage Requirements	Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Assay Range	15.60 to 1000 pg/mL
For Use With (Application)	ELISA

222

Invitrogen™ Human/Mouse TGF beta-1 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human,Mouse
Conjugate	HRP
Gene Symbol	Tgfb1
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
Assay Sensitivity	15.6 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P01137, P04202
Regulatory Status	RUO
Target	TGF beta-1
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21803, 7040
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Transforming Growth Factor beta 1, TGF-b1

223

Invitrogen™ Mouse IL-10 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	IL-1 beta,Il-1 b
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	32 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	32 to 4,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P18893
Regulatory Status	RUO
Target	IL-10
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16153
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin 10, CSIF, Cytokine Synthesis Inhibitory Factor

224

Invitrogen™ ProcartaPlex™ Human Cell Death Panel, 4plex

The ProcartaPlex Human Cell Death Panel 4plex enables cancer research by analyzing four protein targets involved in cell death using Luminex xMAP technology.

Pricing & Availability

225

Invitrogen™ Mouse IL-22 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	IL22
Total Assay Time	24 hr 30 min
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	15.6 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	Q9JJY9
Regulatory Status	RUO
Target	IL-22
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	50929
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-22

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R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit